Identification and Quantification of Bis-GMA and Teg-DMA Released from Dental Materials by HPLC

Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis-GMA) and triethylenglycol dimethacrylate (Teg-DMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytical methods. We studied residual Bis-GMA and Teg-DMA after in vitro polymerization by high performance liquid chromatography (HPLC) after 24 hr, 7, and 14 days. For quantitative analysis, an RP column (250 4.6mm; 5mm) as stationary phase and isocratic elution with CH3CN :H2O (60 : 40) was used. A calibration curve was constructed using standard solutions of Bis-GMA and Teg-DMA in the concentration range from 1 10 to 5 10mol/L. The limits of detection (LOD) and quantification (LOQ) for both analytes were determined (for Bis-GMA: 0.0076 and 0.0229mmol/L; and for TegDMA: 0.0123 and 0.0373mmol/L, respectively). The accuracy of the method was confirmed by standard additions. The HPLC analysis of residual components revealed that Bis-GMA was released from commercial samples in the highest concentration after 14 days. Teg-DMA was detected only in one of all analyzed dental materials.